The simple sample preparation and ease of use make it practical for the routine clinical laboratory. The HPLC assay demonstrates excellent linearity, acceptable accuracy and precision, and good agreement with a validated ECL method.

Comparisons of results demonstrated good agreement between HPLC and ECL methods ( R 2 = 0.883). The mean was 37.875 ng/ml, the standard deviation was 0.22, and the coefficient of variation was 0.58%. Intra-assay precision was determined by extracting and quantifying 10 serum replicates from one patient. The method was linear for the lower limit of quantification from 3 ng/l up to at least 200 ng/l for 25(OH)D3, with the following equation for the regression line: y = 0.172 X + 2.45 ( R 2 = 0.989). Our HPLC results were compared with a validated electrochemiluminescence method. To assess its analytical performance, we used the RECIPE HPLC Complete Kit and an HPLC-UV instrument. It is also supplied in the diet and plays a pivotal role in calcium homeostasis and skeletal metabolism throughout life. Vitamin D is a fat-soluble steroid hormone precursor that is mainly produced in the skin by exposure to sunlight. In the UK, our skin isn't able to make vitamin D from winter sunlight (November to March) as the sunlight hasn't got enough UVB (ultraviolet B) radiation, making vitamin D supplements even more important.The aim of the present study was to develop a robust and easy to use high performance liquid chromatography (HPLC) to analyze 25(OH)D3 in human serum. Also known as the sunshine vitamin, Vitamin D is produced by the skin when in contact with sunlight.Vitamin D contributes to normal blood Calcium levels, the maintenance of normal bones and teeth, normal muscle function, normal function of the immune system and is also involved in the process of cell division.Vitamin D helps contribute to several important functions in the body and regulates the amount of Calcium and Phosphate needed to keep bones and teeth healthy.

What Are the Benefits of These Liquid Drops?

Supports Healthy Bones and Muscle Function.

Our orange flavoured liquid Vitamin D3 drops absorb quickly and are ideal for those who don’t like to swallow tablets or capsules. Holland & Barrett Vitamin D Liquid Drops support bones, muscles, and immunity.

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